The 5'-flanking sequence of SPSⅢwas cloned from sugarcane genome DNA.
3.从甘蔗基因组中克隆扩增出先前未知的SPSⅢ基因5'侧翼序列。
参考来源 - 甘蔗蔗糖磷酸合成酶基因及其5’侧翼序列的克隆与鉴定Amplified the flanking sequence by using inverse PCR, We have obtained 4 flanking sequence of 4 mutants.
7、采用反向PCR方法扩增，获得4个突变体的旁邻序列。
参考来源 - 水稻突变体库的构建和旁邻序列分析A retroviral vector, called pDAM3, containing the neomycin resistant gene and the antisense human c-myc gene fragment (the third exon and 3'flanking sequence) has been constructed. pDAM3 was introduced into PA317 amphotropic packaging cells by the calcium phosphate precipitation method.
兹将实验简介如下：1．构建了一个含有反义人c-myc基因片段(第三外显子及3′旁侧序列)的逆转录病毒载体p-DAM3。
参考来源 - 逆转录病毒载体介导的反义人c·2,447,543篇论文数据，部分数据来源于NoteExpress
The result showed that this IPCR technique is quick, convenient and stable for flanking sequence cloning.
实验结果表明这个方法具有快速、稳定和高效的优点。
In modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
在现代分子生物学技术研究中，常常需要对已知位点的侧翼序列进行分析或克隆，以研究基因的遗传表达调控。
On the basis of inverse PCR, we have founded a IPCR technique to clone foreign gene's flanking sequence in transgenic rice, which is suitable to treat mass materials.
以反向pcr (IPCR)为基础建立了适合于处理大量材料的克隆转基因水稻中外源基因旁侧序列的技术体系。
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